Misplaced Pages

Optimal cutting temperature compound

Article snapshot taken from Wikipedia with creative commons attribution-sharealike license. Give it a read and then ask your questions in the chat. We can research this topic together.
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production

Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a sample onto slides for analysis.

Components

From MSDS:

  • 5-15% polyvinyl alcohol
  • 1-10% polyethylene glycol
  • 75-95% non-reactive ingredients

See also

References

  1. "Safety Data Sheet" (PDF). Sakura. Retrieved 26 November 2024.

Further reading

  • Turbett, Gavin; Sellner, Loryn (1997). "The use of optimal cutting temperature compound can inhibit amplification by polymerase chain reaction". Diagnostic Molecular Pathology. 6 (5): 298–303. doi:10.1097/00019606-199710000-00009. PMID 9458390.


Stub icon

This article related to pathology is a stub. You can help Misplaced Pages by expanding it.

Categories: