Pacelli is an Italian surname. Notable people with the surname include:
- Asprilio Pacelli (1570–1623), Italian Baroque composer
- Ernesto Pacelli (died 1925), Vatican financial adviser, cousin of Pius XII
- Eugenio Pacelli (1876–1958), Pope Pius XII
- Francesco Pacelli (1872–1935), Vatican lawyer, elder brother of Pius XII
- Frank Pacelli (1924–1997), American TV personality
- Vincent "Vinny Basile" Pacelli, American Mafioso indicted in Operation Old Bridge
- William V. Pacelli (1893–1942), American politician
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