Pierlot is a French surname. Notable people with the surname include:
- Hubert Pierlot (1883–1963), Belgian Prime Minister between 1939 and 1945
- Francis Pierlot (1875–1955), American film actor
- Philippe Pierlot (born 1958), Belgian viol player and conductor
- Pierre Pierlot (1921–2007), French classical oboist
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