Ratso may refer to:
- "Ratso" Rizzo, a main character in the film Midnight Cowboy and the novel on which it is based
- nickname of John Hiller (born 1943), Canadian former Major League Baseball relief pitcher
- nickname of Larry Sloman, American author best known for his collaboration with Howard Stern
- Ratso, a character on the 2000-2005 American animated TV show Jackie Chan Adventures
- Ratso, a character on the 1988-1989 anime Wowser
- Ratso the Rat, a character on the 1981 Saturday morning cartoon The Kwicky Koala Show
- Ratso, a character on the 1970-1971 American animated TV show Groovie Goolies
- Ratso, a character in The Brave Little Toaster to the Rescue and The Brave Little Toaster Goes to Mars
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