Rouillier is a French-origin surname. Notable persons with the surname and associated entities include:
- Chase Roullier (born 1993), American football player
- Groupe Roullier, a French agribusiness founded by Daniel Roullier
- Karl Rouillier (1814-1858), Russian zoologist and paleontologist
- Tim Ryan Rouillier (born 1964), American country singer
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