SCHEMA is a computational algorithm used in protein engineering to identify fragments of proteins (called schemas) that can be recombined without disturbing the integrity of the proteins' three-dimensional structure. The algorithm calculates the interactions between a protein's different amino acid residues to determine which interactions may be disrupted by swapping structural domains of the protein. By minimizing these disruptions, SCHEMA can be used to engineer chimeric proteins that stably fold and may have altered function relative to their parent proteins. SCHEMA algorithm has been applied in the recombinant libraries of distantly related β-lactamases.
References
- Voigt, CA; Martinez, C; Wang, ZG; Mayo, SL; Arnold, FH; et al. (June 2002). "Protein building blocks preserved by recombination". Nature Structural Biology. 9 (7): 553–558. doi:10.1038/nsb805. PMID 12042875. S2CID 19170079.
- Otey, CR; Landwehr, M; Endelman, JB; Hiraga, K; Bloom, JD; Arnold, FH (May 2006). "Structure-guided recombination creates an artificial family of cytochromes P450". PLOS Biology. 4 (5): e112. doi:10.1371/journal.pbio.0040112. PMC 1431580. PMID 16594730.
- Meyer, M; Hochrein, L.; Arnold, F (2006). "Structure-guided SCHEMA recombination of distantly related beta-lactamases". Protein Eng Des Sel. 19 (12): 563–570. doi:10.1093/protein/gzl045. PMID 17090554.