San Sebastiano can refer to:
- Saint Sebastian in Italian
- San Sebastiano Curone, a comune in the Italian region Piedmont
- San Sebastiano da Po, a comune in the Italian region Piedmont
- San Sebastiano al Vesuvio, a settlement destroyed by the 1944 eruption of Mount Vesuvius, but since rebuilt.
- San Sebastiano (Biella), a church in Biella, Italy
- San Sebastiano (Milan), a church in Milan, Italy
- San Sebastiano (Mantua), a church in Mantua, Italy
- San Sebastiano fuori le mura, a church in Rome, Italy
- San Sebastiano al Palatino, a church in Rome, Italy
- San Sebastiano, Venice, a church in Venice, Italy
- San Sebastiano, Verona, a former church in Verona, Italy
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