Serio may refer to:
- Sério, a municipality in Rio Grande do Sul, Brazil
- Serio (river), a river in Lombardy, Italy
- 11022 Serio, a main belt asteroid
People with the surname
- José Sério (1922–2010), Portuguese footballer
- Renato Serio (born 1946), Italian composer, conductor and arranger
- Steve Serio (born 1987), American wheelchair basketball player
Fictional
- Serio (HMX-13), a character from the visual novel To Heart
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