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Sf9 (cells)

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Insect cell line
Phase contrast image of SF9 cells

Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.

Sf9 Rhabdovirus

It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.

References

  1. Vaughn JL; Goodwin RH; Tompkins GJ; McCawley P (1977). "The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae)". In Vitro. 13 (4): 213–217. doi:10.1007/BF02615077. PMID 68913. S2CID 13529129.
  2. Invitrogen (14 Mar 2017). "Cell Lines". Growth and Maintenance of Insect cell lines: User Guide (PDF). Rev. Date - 8 March 2010. Invitrogen. Manual part no. 25-0127, MAN0000030. Retrieved 12 May 2017.
  3. Ma H, Galvin TA, Glasner DR, Shaheduzzaman S, Khan AS (2014). "Identification of a novel rhabdovirus in Spodoptera frugiperda cell lines". J Virol. 88 (12): 6576–85. doi:10.1128/JVI.00780-14. PMC 4054387. PMID 24672045.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  4. Parry R, de Malmanche H, Asgari S (2021). "Persistent Spodoptera frugiperda rhabdovirus infection in Sf9 cells is not restricted by Wolbachia wMelPop-CLA and wAlbB strains and is targeted by the RNAi machinery". Virology. 563: 82–87. doi:10.1016/j.virol.2021.08.013. PMID 34492433.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  5. Hashimoto Y, Macri D, Srivastava I, McPherson C, Felberbaum R, Post P; et al. (2017). "Complete study demonstrating the absence of rhabdovirus in a distinct Sf9 cell line". PLOS ONE. 12 (4): e0175633. Bibcode:2017PLoSO..1275633H. doi:10.1371/journal.pone.0175633. PMC 5397025. PMID 28423032.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  6. Maghodia AB, Jarvis DL (2017). "Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines". Virology. 512: 234–245. doi:10.1016/j.virol.2017.09.025. PMC 5651996. PMID 29024851.

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