Spiritus (Latin for "spirit" or "breathing") may refer to:
- Spiritus lenis, the "soft breathing" in Byzantine Greek orthography
- Spiritus asper, the "hard breathing" in Byzantine Greek orthography
- Spiritus (journal), an academic journal devoted to the study of Christian spirituality
- Spiritus (novel), a 1996 novel by Ismail Kadare
- Spiritus (EP) an extended play by Australian singer songwriter, Lisa Mitchell, 2012
- "Spiritus" (song) a single by Lisa Mitchell from the 2012 EP
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