Sulphide Indole Motility medium

Sulphide Indole Motility (SIM) medium is a bacterial growth medium which tests for the ability to reduce sulfates, the ability to produce indoles, and motility. This combination of challenges in one mixture is convenient and commercially available in stab tubes. Inoculated needles are then punctured into the culture and incubated, if the culture becomes cloudy the bacteria were able to infiltrate the media and survive. This method is particularly useful for pathogenic bacteria which are dangerous to handle on wet mount slides

References

"New Page 1". faculty.lacitycollege.edu. Retrieved 2016-12-07.
"Tests for Bacterial Motility: Procedure and Results - microbeonline". microbeonline. 2015-10-19. Retrieved 2016-12-07.
"Motility Test (Theory) : Microbiology Virtual Lab I : Biotechnology and Biomedical Engineering : Amrita Vishwa Vidyapeetham Virtual Lab". vlab.amrita.edu. Retrieved 2016-12-07.

Growth media / agar plates

Selective media

Gram positive

Actinomycetota	Mycobacterium tuberculosis Löwenstein–Jensen medium Middlebrook 7H9 Broth Middlebrook 7H10 Agar Middlebrook 7H11 Agar Mycoplasma pneumoniae Eaton's agar
Bacillota	Corynebacterium diphtheriae Hoyle's agar Enterococcus Bile esculin agar Lactobacillus Lee's Agar MRS agar Lactococcus M17 agar Listeria Fraser broth PALCAM Staphylococcus Mannitol salt agar Baird-Parker agar Vogel–Johnson agar

Gram negative

Alphaproteobacteria	Brucella Brucella agar Farrell's medium
Betaproteobacteria	Neisseria Thayer–Martin agar New York City agar
Gammaproteobacteria	Aggregatibacter actinomycetemcomitans Tryptic soy-serum-bacitracin-vancomycin Bordetella Bordet–Gengou agar Enterobacteriaceae VRBD agar Haemophilus influenzae/Legionella pneumophila Buffered charcoal yeast extract agar Pseudomonas aeruginosa Cetrimide agar Salmonella XLT agar DCA agar Salmonella/Shigella XLD agar

Differential media

Fungal media

Nonselective media

Other/ungrouped media

Techniques in clinical microbiology

Isolation
and culture

Isolation techniques	Asepsis Streak plate Selective media
Cultures by body site	Blood culture Genital culture Sputum culture Throat culture Urine culture Wound culture
Cultures by organism	Bacterial culture Fungal culture Viral culture

Identification
and testing

Manual testing: basic techniques	Colonial morphology Hemolysis Staining Gram stain Acid-fast stain Giemsa stain India ink stain Ziehl–Neelsen stain Wet prep Rapid tests Oxidase Catalase Indole PYR
Manual testing: biochemical and immunologic tests	ALA test Amino acid decarboxylase test Bile solubility test CAMP test Citrate test Coagulase test DNAse test IMViC KOH test Methyl red test Nitrite test ONPG test Oxidative/fermentation glucose test Phenylalanine deaminase test Reverse CAMP test Salt tolerance test Sulfide indole motility test Triple sugar iron test Urease test rapid Voges–Proskauer test X and V factor test Bacitracin susceptibility test Optochin susceptibility test Novobiocin susceptibility test Lancefield grouping RPR test
Automated and point-of-care testing	Analytical profile index MALDI-TOF Polymerase chain reaction VITEK Rapid strep test Monospot test
Antibiotic susceptibility testing	Beta-lactamase test Disk diffusion test Etest McFarland standards Minimum inhibitory concentration

Equipment

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