tRNA (guanine37-N1)-methyltransferase

tRNA (guanine-N)-methyltransferase (EC 2.1.1.228, TrmD, tRNA (m1G37) methyltransferase, transfer RNA (m1G37) methyltransferase, Trm5p, TRMT5, tRNA-(N1G37) methyltransferase, MJ0883 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine-N)-methyltransferase. This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + guanine in tRNA

\rightleftharpoons

S-adenosyl-L-homocysteine + N-methylguanine in tRNA

This enzyme is important for the maintenance of the correct reading frame during translation.

References

Takeda H, Toyooka T, Ikeuchi Y, Yokobori S, Okadome K, Takano F, Oshima T, Suzuki T, Endo Y, Hori H (December 2006). "The substrate specificity of tRNA (mG) methyltransferase (TrmD) from Aquifex aeolicus". Genes to Cells. 11 (12): 1353–65. doi:10.1111/j.1365-2443.2006.01022.x. PMID 17121543.
Lee C, Kramer G, Graham DE, Appling DR (September 2007). "Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase". The Journal of Biological Chemistry. 282 (38): 27744–53. doi:10.1074/jbc.m704572200. PMID 17652090.
O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brulé H, Petit C, Holmes DJ, Zalacain M, Holmes WM (April 2004). "Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth". Journal of Bacteriology. 186 (8): 2346–54. doi:10.1128/jb.186.8.2346-2354.2004. PMC 412112. PMID 15060037.
Brulé H, Elliott M, Redlak M, Zehner ZE, Holmes WM (July 2004). "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein". Biochemistry. 43 (28): 9243–55. doi:10.1021/bi049671q. PMID 15248782.
Goto-Ito S, Ito T, Ishii R, Muto Y, Bessho Y, Yokoyama S (September 2008). "Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5". Proteins. 72 (4): 1274–89. doi:10.1002/prot.22019. PMID 18384044.
Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK (June 2003). "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition". The EMBO Journal. 22 (11): 2593–603. doi:10.1093/emboj/cdg269. PMC 156765. PMID 12773376.

External links

TRNA+(guanine37-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase
O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase
Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase
Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase Cyclopropane-fatty-acyl-phospholipid synthase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase
Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase
Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase
Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 2.1.1