Topknot may refer to:
- A hairstyle or haircut, historically prevalent in Asia:
- Chonmage, a traditional Japanese haircut worn by men
- Sangtu (상투), a Korean topknot
- Touji (頭髻), a traditional Chinese hairstyle which involves tying all hair into a bun, worn from earliest times up to the end of the Ming Dynasty and still worn by Taoist priests and practitioners
- Shikha, worn by orthodox Hindu priests
- joora, worn by Sikh men
- Khokhol, oseledets traditional Ukrainian haircut
- The Suebian knot, is a historical male hairstyle ascribed to the tribe of the Germanic Suebi
- Tikitiki, a top-knot worn by high-ranking Māori men
- Sidelock of youth worn by pharaonic children in Middle-Kingdom Egypt, indicating association with the child Horus
- Man bun, a modern Western style of topknot influenced by the Asian style, a trend beginning in the 2010s
- Several species of fishes:
- The New Zealand topknot, Notoclinus fenestratus
- The Norwegian topknot, Phrynorhombus norvegicus
- The Brown topknot, Notoclinus compressus
- Several species in the genus Zeugopterus
- Topknot pigeon, Lopholaimus antarcticus
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