Torulene dioxygenase

Torulene dioxygenase (EC 1.13.11.59, CAO-2, CarT) is an enzyme with systematic name torulene:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction

\rightleftharpoons

4'-apo-beta, psi-caroten-4'-al + 3-methylbut-2-enal

It is assumed that 3-methylbut-2-enal is formed.

References

Prado-Cabrero A, Estrada AF, Al-Babili S, Avalos J (April 2007). "Identification and biochemical characterization of a novel carotenoid oxygenase: elucidation of the cleavage step in the Fusarium carotenoid pathway". Molecular Microbiology. 64 (2): 448–60. doi:10.1111/j.1365-2958.2007.05665.x. PMID 17493127.
Saelices L, Youssar L, Holdermann I, Al-Babili S, Avalos J (November 2007). "Identification of the gene responsible for torulene cleavage in the Neurospora carotenoid pathway". Molecular Genetics and Genomics. 278 (5): 527–37. doi:10.1007/s00438-007-0269-2. PMID 17610084.
Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S (November 2008). "Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation". Fungal Genetics and Biology. 45 (11): 1497–505. doi:10.1016/j.fgb.2008.09.001. PMID 18812228.

Torulene+dioxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Oxidoreductases: monooxygenases (EC 1.13)
1.13.11: two atoms of oxygen	lipoxygenase: Arachidonate 5-lipoxygenase Arachidonate 12-lipoxygenase/ALOX12 Arachidonate 8-lipoxygenase Arachidonate 15-lipoxygenase/ALOX15 Linoleate 11-lipoxygenase other dioxygenase: Catechol dioxygenase Homogentisate 1,2-dioxygenase Cysteine dioxygenase 4-Hydroxyphenylpyruvate dioxygenase Indoleamine 2,3-dioxygenase Chlorite dismutase
1.13.12: one atom of oxygen	Firefly luciferase
1.13.99: other	Inositol oxygenase

1.13.11: two atoms of oxygen

1.13.12: one atom of oxygen

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)