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Tsukubusuma Shrine (都久夫須麻神社, Tsukubusuma Jinja) is a Shinto shrine on Chikubu Island in Shiga Prefecture, Japan. Its main hall (本殿, honden) is a National Treasure of Japan. The shrine's main festival is held annually on June 15. It is also called Chikubushima Shrine (竹生島神社, Chikubushima Jinja).