Tsukuyomi may refer to:
- Tsukuyomi-no-Mikoto (月読), a moon god in Shinto and Japanese mythology
- 10412 Tsukuyomi, an asteroid from the asteroid belt.
- Tsukuyomi: Moon Phase, a manga and anime series
- Tsukuyomi (月読, "moon reader"), a jutsu used by Itachi Uchiha in the Naruto manga and anime series
- Tsukuyomi (Negima! Magister Negi Magi), a character in the manga series Negima!
- Ikuto Tsukiyomi, a fictional character from the manga series Shugo Chara! by Peach-Pit. Older brother of Utau Tsukiyomi
- Utau Tsukiyomi, a fictional character from the manga series Shugo Chara! by Peach-Pit. Younger sister of Ikuto Tsukiyomi
- Komoe Tsukuyomi, a fictional character from the light novel Toaru Majutsu no Index.
- Tsukuyomi, a Yu-Gi-Oh! card monster created by Kazuki Takahashi.
- Tsukuyomi, a fictional character from Tsubasa: Reservoir Chronicle whose real name is Princess Tomoyo. She has an older sister whose name is Amaterasu.
- Tsukuyomi, a fictional character from the anime series My Hero Academia, The Jet-Black Hero, Tsukuyomi is the alias of Fumikage Tokoyami
- Tsukuyomi (月詠み) [ja], a Japanese rock band and musical project by Yurry Canon [ja] under Speedstar Records [ja]
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