Ustajlu is a surname. Notable people with the surname include:
- Abdollah Khan Ustajlu (died 1566/67), Iranian dignitary of Turkoman origin
- Badr Khan Ustajlu, Turkoman military officer
- Evaz Beg Ustajlu, Turkoman military officer
- Mohammad Khan Ustajlu (died 1514), Iranian military commander
- Mohammad Khan Tokhmaq Ustajlu, 16th-century Iranian official
- Salman Khan Ustajlu, Turkoman military leader
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