Vent DNA polymerase - Misplaced Pages

See also: Taq polymerase, Pfu DNA polymerase, Pwo DNA polymerase, and Variants of PCR

DNA polymerase

Identifiers

Organism

Thermococcus litorali

Symbol

pol

UniProt

P30317

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Structures	Swiss-model
Domains	InterPro

Vent polymerase is a archean thermostable DNA polymerase used for the polymerase chain reaction. It was isolated from the thermophile Thermococcus litoralis.

References

Vigneault F, Drouin R (2005). "Optimal conditions and specific characteristics of Vent exo- DNA polymerase in ligation-mediated polymerase chain reaction protocols". Biochem Cell Biol. 83 (2): 147–65. doi:10.1139/o04-134. PMID 15864324.

Transferases: phosphorus-containing groups (EC 2.7)

2.7.1-2.7.4:
phosphotransferase/kinase
(PO₄)

2.7.1: OH acceptor	Hexo- Gluco- Fructo- Hepatic Galacto- Phosphofructo- 1 Liver Muscle Platelet 2 Riboflavin Shikimate Thymidine ADP-thymidine NAD Glycerol Pantothenate Mevalonate Pyruvate Deoxycytidine PFP Diacylglycerol Phosphoinositide 3 Class I PI 3 Class II PI 3 Sphingosine Glucose-1,6-bisphosphate synthase
2.7.2: COOH acceptor	Phosphoglycerate Aspartate kinase
2.7.3: N acceptor	Creatine
2.7.4: PO₄ acceptor	Phosphomevalonate Adenylate Nucleoside-diphosphate Uridylate Guanylate Thiamine-diphosphate

2.7.6: diphosphotransferase
(P₂O₇)

2.7.7: nucleotidyltransferase
(PO₄-nucleoside)

Polymerase

DNA polymerase

DNA-directed DNA polymerase

I/A

Taq

II/B

Pfu

III/C

IV/X

TDT

V/Y

RNA-directed DNA polymerase

Reverse transcriptase

Telomerase

RNA polymerase

Template-directed

RNA-dependent RNA polymerase

Polyadenylation

PAP

PNPase

Phosphorolytic
3' to 5' exoribonuclease

Nucleotidyltransferase

Guanylyltransferase

mRNA capping enzyme

Other

2.7.8: miscellaneous

Phosphatidyltransferases	CDP-diacylglycerol—glycerol-3-phosphate 3-phosphatidyltransferase CDP-diacylglycerol—serine O-phosphatidyltransferase CDP-diacylglycerol—inositol 3-phosphatidyltransferase CDP-diacylglycerol—choline O-phosphatidyltransferase
Glycosyl-1-phosphotransferase	N-acetylglucosamine-1-phosphate transferase

2.7.10-2.7.13: protein kinase
(PO₄; protein acceptor)

2.7.10: protein-tyrosine	see tyrosine kinases
2.7.11: protein-serine/threonine	see serine/threonine-specific protein kinases
2.7.12: protein-dual-specificity	see serine/threonine-specific protein kinases
2.7.13: protein-histidine	Protein-histidine pros-kinase Protein-histidine tele-kinase Histidine kinase

v t e Polymerase chain reaction techniques
Procedure	Initialization Cycle Denaturation Annealing Elongation Final elongation Final hold
Polymerase	Klenow T4 and T7 Taq Tth Pfu Vent Pwo Tli Tfu
Optimization and variants	Quantitative polymerase chain reaction (qPCR) Reverse transcription polymerase chain reaction (RT-PCR) Inverse polymerase chain reaction (inverse PCR) Nested polymerase chain reaction (nested PCR) Touchdown polymerase chain reaction Hot start PCR Overlap extension polymerase chain reaction (OE-PCR) Multiplex polymerase chain reaction (multiplex PCR) Multiplex ligation-dependent probe amplification (MLPA) Co-amplification at lower denaturation temperature PCR (COLD-PCR) Digital polymerase chain reaction (dePCR) Random amplification of polymorphic DNA (RAPD)
History and people	Kary Mullis Kjell Kleppe Alice Chien

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