WLS may refer to:
Arts, entertainment, and media
- WLS (AM), a radio station in Chicago, Illinois, US
- WLS-FM, a radio station in Chicago, Illinois, US
- WLS-TV, a television station in Chicago, Illinois, US
- DWLS, a radio station in Metro Manila, Philippines
Biology and healthcare
- GPR177, Wntless, or WLS, a human gene
- Weight loss surgery
Computing and technology
- Oracle WebLogic Server, a Java application server
- White light scanner, for measuring surface height
- Wavelength shifter, material that absorbs a wavelength and emits another
Other uses
- Wisconsin Lutheran Seminary, US
- WLS, the Chapman code for Wales
- Weighted least squares, in statistics
- West London Synagogue
- Westchester Library System, New York, US
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