Wright's stain - Misplaced Pages

(Redirected from Wright stain) Hematologic stain that facilitates the visual differentiation of blood cell types

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Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope. In cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.

It is named for James Homer Wright, who devised the stain, a modification of the Romanowsky stain, in 1902. Because it distinguishes easily between blood cells, it became widely used for performing differential white blood cell counts, which are routinely ordered when conditions such as infection or leukemia are suspected.

The related stains are known as the buffered Wright stain, the Wright-Giemsa stain (a combination of Wright and Giemsa stains), and the buffered Wright-Giemsa stain, and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue (some commercial preparations combine solutions to simplify staining). The May–Grünwald stain, which produces a more intense coloration, also takes a longer time to perform.

Urine samples stained with Wright's stain will identify eosinophils, which can indicate interstitial nephritis or urinary tract infection.

White blood cells stained with Wright's stain:

Lymphocyte

Basophil

Neutrophil

References

"Wright Giemsa Stains". Sigma-Aldrich. Retrieved 2016-10-28.
Corwin, H. L.; Bray, R. A.; Haber, M. H. (1989-11-01). "The detection and interpretation of urinary eosinophils". Archives of Pathology & Laboratory Medicine. 113 (11): 1256–1258. ISSN 0003-9985. PMID 2479358.

v t e Microbial and histological stains
Iron/hemosiderin	Perls Prussian blue
Lipids	Sudan stain Sudan II Sudan III Sudan IV Oil Red O Sudan Black B
Carbohydrates	Alcian blue Mucicarmine Periodic acid–Schiff stain
Amyloid	Congo red Thioflavin
Bacteria	Gram stain Methyl violet/Gentian violet Safranin Acid-fast Ziehl–Neelsen stain/Kinyoun stain Carbol fuchsin/Fuchsine Methylene blue Auramine–rhodamine stain Auramine O Rhodamine B Auramine phenol stain
Connective tissue	trichrome stain: Masson's trichrome stain/Lillie's trichrome Light Green SF yellowish Biebrich scarlet Phosphomolybdic acid Fast Green FCF Sirius Red Van Gieson's stain
Other	Cresyl violet Cyanine Jaswant Singh–Bhattacharji (JSB) stain H&E stain Haematoxylin Eosin Y Janus Green B Giemsa stain Gömöri trichrome stain Luxol fast blue stain Methyl blue Moeller stain Movat's stain Neutral red Schaeffer–Fulton stain Silver stain Bielschowsky stain Grocott's methenamine silver stain Warthin–Starry stain Wright's stain
Tissue stainability	Acidophilic Basophilic Chromophobic

v t e Romanowsky stains
Diff-Quik Field stain Jaswant Singh–Bhattacharji stain Jenner's stain Leishman stain May–Grünwald stain Wright stain

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References