Desosaminyl transferase EryCIII

Desosaminyl transferase EryCIII (EC 2.4.1.278, EryCIII) is an enzyme with systematic name dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase. This enzyme catalyses the following chemical reaction

dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B

\rightleftharpoons

dTDP + erythromycin D

The enzyme is involved in erythromycin biosynthesis.

References

Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S (October 2005). "In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides". Journal of the American Chemical Society. 127 (41): 14128–9. doi:10.1021/ja053704n. PMC 2525786. PMID 16218575.
Lee HY, Chung HS, Hang C, Khosla C, Walsh CT, Kahne D, Walker S (August 2004). "Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway". Journal of the American Chemical Society. 126 (32): 9924–5. doi:10.1021/ja048836f. PMID 15303858.
Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF (January 2012). "Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII". Journal of Molecular Biology. 415 (1): 92–101. doi:10.1016/j.jmb.2011.10.036. PMC 3391682. PMID 22056329.

External links

Desosaminyl+transferase+EryCIII at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Transferases: glycosyltransferases (EC 2.4)

2.4.1: Hexosyl-
transferases

Glucosyl-	Phosphorylase Starch Glycogen Cellobiose Myo- Glycogen synthase Debranching enzyme Branching enzyme 1,3-Beta-glucan synthase Ceramide glucosyltransferase N-glycosyltransferase
Galactosyl-	Lactose synthase B-N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase (C1GALT1)
Glucuronosyl-	B3GAT1 B3GAT2 B3GAT3 UGT1A1 UGT1A3 UGT1A4 UGT1A5 UGT1A6 UGT1A7 UGT1A8 UGT1A9 UGT1A10 UGT2A1 UGT2A2 UGT2A3 UGT2B4 UGT2B7 UGT2B10 UGT2B11 UGT2B15 UGT2B17 UGT2B28 Hyaluronan synthase: HAS1 HAS2 HAS3
Fucosyl-	POFUT1 POFUT2 FUT1 FUT2 FUT3 FUT4 FUT5 FUT6 FUT7 FUT8 FUT9 FUT10 FUT11
Mannosyl-	Dolichyl-phosphate-mannose-protein mannosyltransferase POMT1 POMT2 DPM1 DPM3 ALG1 ALG2 ALG3 ALG6 ALG8 ALG9 ALG12

2.4.2: Pentosyl-
transferases

Ribose

ADP-ribosyltransferase

NAD:diphthamide ADP-ribosyltransferase
- Diphtheria toxin
- Pseudomonas exotoxin

NAD(P):arginine ADP-ribosyltransferase
- Pertussis toxin
- Cholera toxin

Poly ADP ribose polymerase

Sirtuin

Phosphoribosyltransferase

Other

Purine nucleoside phosphorylase: Thymidine phosphorylase
- ECGF1

Other

2.4.99: Sialyl
transferases

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 2.4.1