Trans-o-hydroxybenzylidenepyruvate hydratase-aldolase

trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase (EC 4.1.2.45, 2′-hydroxybenzalpyruvate aldolase, NsaE, tHBPA hydratase-aldolase) is an enzyme with systematic name (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase. This enzyme catalyses the following chemical reaction

(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H₂O

\rightleftharpoons

salicylaldehyde + pyruvate

This enzyme is involved in naphthalene degradation.

References

Kuhm AE, Knackmuss HJ, Stolz A (May 1993). "Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates". The Journal of Biological Chemistry. 268 (13): 9484–9. PMID 8486638.
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J (July 2006). "Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6". Microbiology. 152 (Pt 7): 1929–40. doi:10.1099/mic.0.28783-0. PMID 16804169.
Eaton RW (December 1994). "Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid". Journal of Bacteriology. 176 (24): 7757–62. doi:10.1128/jb.176.24.7757-7762.1994. PMC 197239. PMID 8002605.
Eaton RW (June 2000). "trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst". Applied and Environmental Microbiology. 66 (6): 2668–72. doi:10.1128/aem.66.6.2668-2672.2000. PMC 110598. PMID 10831455.

External links

Trans-o-hydroxybenzylidenepyruvate+hydratase-aldolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Carbon–carbon lyases (EC 4.1)
4.1.1: Carboxy-lyases	Acetoacetate decarboxylase Adenosylmethionine decarboxylase Arginine decarboxylase Aromatic L-amino acid decarboxylase Glutamate decarboxylase Histidine decarboxylase Lysine decarboxylase Malonyl-CoA decarboxylase Ornithine decarboxylase Oxaloacetate decarboxylase Phosphoenolpyruvate carboxykinase Phosphoenolpyruvate carboxylase Phosphoribosylaminoimidazole carboxylase Pyrophosphomevalonate decarboxylase Pyruvate decarboxylase RuBisCO Uridine monophosphate synthetase/Orotidine 5'-phosphate decarboxylase Uroporphyrinogen III decarboxylase
4.1.2: Aldehyde-lyases	Fructose-bisphosphate aldolase Aldolase A Aldolase B Aldolase C 2-hydroxyphytanoyl-CoA lyase Threonine aldolase
4.1.3: Oxo-acid-lyases	Isocitrate lyase 3-hydroxy-3-methylglutaryl-CoA lyase
4.1.99: Other	Tryptophanase Photolyase CPD lyase Spore photoproduct lyase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 4.1.2