Misplaced Pages

In biochemistry, tyrosine sulfation is a posttranslational modification where a sulfate group (−SO₃) is added to a tyrosine residue of a protein molecule. Secreted proteins and extracellular parts of membrane proteins that pass through the Golgi apparatus may be sulfated. Sulfation was first discovered by Bettelheim in bovine fibrinopeptide B in 1954 and later found to be present in animals and plants but not in prokaryotes or in yeast.

Function

Sulfation plays a role in strengthening protein-protein interactions. Types of human proteins known to undergo tyrosine sulfation include adhesion molecules, G-protein-coupled receptors, coagulation factors, serine protease inhibitors, extracellular matrix proteins, and hormones. Tyrosine O-sulfate is a stable molecule and is excreted in urine in animals. No enzymatic mechanism of tyrosine sulfate desulfation is known to exist.

By knock-out of TPST genes in mice, it may be observed that tyrosine sulfation has effects on the growth of the mice, such as body weight, fecundity, and postnatal viability.

Mechanism

Sulfation is catalyzed by tyrosylprotein sulfotransferase (TPST) in the Golgi apparatus. The reaction catalyzed by TPST is a transfer of sulfate from the universal sulfate donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to the side-chain hydroxyl group of a tyrosine residue. Sulfation sites are tyrosine residues exposed on the surface of the protein typically surrounded by acidic residues; a detailed description of the characteristics of the sulfation site was available from PROSITE and predicted by an on-line tool named the Sulfinator. Two types of tyrosylprotein sulftotransferases (TPST-1 and TPST2) have been identified.

Regulation

There is very limited evidence that the TPST genes are subject to transcriptional regulation and tyrosine O-sulfate is very stable and cannot be easily degraded by mammalian sulfatases. Tyrosine O-sulfation is an irreversible process in vivo.

Clinical Significance

It has been shown that the sulfation of Tyr1680 in Factor VIII is essential for effective binding to vWF. Thus, when this is mutated, patients may suffer mild haemophiliac symptoms due to increased turnover. Sulfation of the three tyrosines found in human PSGL-1 not only enhance its binding to P-selectin but also enables its pH-selective binding to the immune checkpoint protein VISTA.

Antibody for detection of tyrosine-sulfated epitopes

In 2006, an article was published in the Journal of Biological Chemistry describing the production and characterization of an antibody called PSG2. This antibody shows exquisite sensitivity and specificity for epitopes containing sulfotyrosine independent of the sequence context.

References

1. Bettelheim, F. R. (1954). "Tyrosine-O-Sulfate in a Peptide from Fibrinogen". Journal of the American Chemical Society. 76 (10): 2838–2839. doi:10.1021/ja01639a073. ISSN 0002-7863.
2. Mehta, AY; Heimburg-Molinaro, J; Cummings, RD; Goth, CK (June 2020). "Emerging patterns of tyrosine sulfation and O-glycosylation cross-talk and co-localization". Current Opinion in Structural Biology. 62: 102–111. doi:10.1016/j.sbi.2019.12.002. PMC 7308222. PMID 31927217.
3. (PROSITE pattern: PS00003)
4. Monigatti, Flavio; Gasteiger, Elisabeth; Bairoch, Amos; Jung, Eva (2002-05-01). "The Sulfinator: predicting tyrosine sulfation sites in protein sequences". Bioinformatics. 18 (5): 769–770. doi:10.1093/bioinformatics/18.5.769. ISSN 1367-4811. PMID 12050077.
5. "Expasy: The Sulfinator - documentation".
6. Leyte, A.; Schijndel, H. B. van; Niehrs, C.; Huttner, W. B.; Verbeet, M. P.; Mertens, K.; Mourik, J. A. van (1991-01-15). "Sulfation of Tyr1680 of human blood coagulation factor VIII is essential for the interaction of factor VIII with von Willebrand factor". Journal of Biological Chemistry. 266 (2): 740–746. doi:10.1016/S0021-9258(17)35234-1. ISSN 0021-9258. PMID 1898735.
7. Sako, Dianne; Comess, Kenneth M.; Barone, Karen M.; Camphausen, Raymond T.; Cumming, Dale A.; Shaw, Gray D. (1995). "A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding". Cell. 83 (2). Elsevier BV: 323–331. doi:10.1016/0092-8674(95)90173-6. ISSN 0092-8674. PMID 7585949.
8. Johnston, Robert J.; et al. (2019-10-23). "VISTA is an acidic pH-selective ligand for PSGL-1". Nature. 574 (7779). Springer Science and Business Media LLC: 565–570. Bibcode:2019Natur.574..565J. doi:10.1038/s41586-019-1674-5. ISSN 0028-0836. PMID 31645726. S2CID 204849372.

• Moore KL (2003). "The biology and enzymology of protein tyrosine O-sulfation". J. Biol. Chem. 278 (27): 24243–6. doi:10.1074/jbc.R300008200. PMID 12730193.
• Hoffhines AJ; Damoc, E; Bridges, KG; Leary, JA; Moore, KL (2006). "Detection and purification of tyrosine-sulfated proteins using a novel anti-sulfotyrosine monoclonal antibody". J. Biol. Chem. 281 (49): 37877–87. doi:10.1074/jbc.M609398200. PMC 1764208. PMID 17046811.

• Post-translational modification