Alpha-D-xyloside xylohydrolase

Identifiers

EC no.

3.2.1.177

Databases

PDB structures

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Alpha-D-xyloside xylohydrolase (EC 3.2.1.177, alpha-xylosidase) is an enzyme. This enzyme catalyses the following chemical reaction

Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.

The enzyme catalyses hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide.

References

Moracci M, Cobucci Ponzano B, Trincone A, Fusco S, De Rosa M, van Der Oost J, Sensen CW, Charlebois RL, Rossi M (July 2000). "Identification and molecular characterization of the first alpha -xylosidase from an archaeon". The Journal of Biological Chemistry. 275 (29): 22082–9. doi:10.1074/jbc.M910392199. PMID 10801892.
Sampedro J, Sieiro C, Revilla G, González-Villa T, Zarra I (June 2001). "Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis". Plant Physiology. 126 (2): 910–20. doi:10.1104/pp.126.2.910. PMC 111180. PMID 11402218.
Crombie HJ, Chengappa S, Jarman C, Sidebottom C, Reid JS (January 2002). "Molecular characterisation of a xyloglucan oligosaccharide-acting alpha-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant 'apoplastic' alpha-D-glucosidases". Planta. 214 (3): 406–13. doi:10.1007/s004250100631. PMID 11859845.
Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC (January 2005). "Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate". The Journal of Biological Chemistry. 280 (3): 2105–15. doi:10.1074/jbc.m410468200. PMID 15501829.
Iglesias N, Abelenda JA, Rodiño M, Sampedro J, Revilla G, Zarra I (January 2006). "Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana". Plant & Cell Physiology. 47 (1): 55–63. doi:10.1093/pcp/pci223. PMID 16267099.
Okuyama M, Kaneko A, Mori H, Chiba S, Kimura A (May 2006). "Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase". FEBS Letters. 580 (11): 2707–11. doi:10.1016/j.febslet.2006.04.025. PMID 16631751.
Larsbrink J, Izumi A, Ibatullin FM, Nakhai A, Gilbert HJ, Davies GJ, Brumer H (June 2011). "Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification" (PDF). The Biochemical Journal. 436 (3): 567–80. doi:10.1042/bj20110299. PMID 21426303.

External links

Alpha-D-xyloside+xylohydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase
Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme
Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Klotho Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

Portal:

Biology

Category:

EC 3.2.1