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Natronomonas (common abbreviation Nmn.) is a genus of the Halobacteriaceae.

Description and significance

Natronomonas pharaonis is an aerobic, extremely haloalkaliphilic archaeon that grows optimally in 3.5M sodium chloride and at pH 8.5, but is sensitive to high magnesium concentrations.

Phylogeny

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI).

Genome structure

The genome of Natronomonas pharaonis consists of three circular replicons, the chromosome which is 2,595,221 bp in length, a typical haloarchaeal 131-kb plasmid, and a unique multicopy 23-kb plasmid. Its chromosome has a high G + C content (63.4%). Also, a high proportion of acidic amino acids (average 19.3%) is found in the proteins of N. pharaonis which results in low isoelectric points (average pI 4.6). This is considered to be one of the adaptive features of haloarchaea, which are known to apply the salt-in strategy (high internal salt concentrations) in order to survive in their hypersaline environment (Falb et al.). Further, it is noteworthy that because the archaeon lacks the genetic encoding for key enzymes for glycolytic pathways, it is not capable of sugar utilization.

Cell structure and metabolism

Natronomonas, like the other members of Halobacteriaceae, has a distinct physiological characteristics because it not only requires high NaCl concentrations but also high pH and low Mg2+ concentrations for growth. It usually utilizes amino acids as the carbon source, but the series of studies discovered that the archaeon has a high degree of nutritional self-sufficiency. Also, in contrast to other alkaliphiles, which use sodium Na+ instead of protons H+ as coupling ion between respiratory chain and ATP synthase, Natronomonas uses protons as coupling ion.

The archaeon grows under highly alkaline conditions of pH around 11, which causes reduced levels of ammonia in addition to low availability of metal ions. The genome analysis shows that, in its nitrogen metabolism process, the archaeon has three mechanisms that supply ammonia, which is then assimilated into glutamate: direct uptake of ammonia, uptake of nitrate and subsequent reduction to ammonia, and uptake of urea which is split by urease to release ammonia. The green arrows in the figure represent the transporters for exogenous nitrogen source ammonia (AmtB), nitrate (NarK), and urea (UrtA-E), and the blue arrows represent the enzymes for reduction of nitrate (NarB + Nir A) and hydrolysis of urea (UreA-G). Other abbreviations: GlnA + GltB = glutamate; 2-OG = oxoglutarate; fdx = ferredoxin.

It is probable that Natronomonas uses ferredoxin and not NADH as the electron donor for all three reductive conversions. This is evident from the occurrence of conserved ferredoxin-binding residues within the N. pharaonis NirA protein and ferredoxin dependence of nitrate and nitrite reductases in the halophile Haloferax mediterranei.

Ecology

Strains of N. pharaonis were first isolated from highly saline soda lakes in Egypt and Kenya, which show pH values around 11.

See also

• List of Archaea genera

References

1. ^ Sayers; et al. "Natronomonas". National Center for Biotechnology Information (NCBI) taxonomy database. Retrieved 2023-10-10.
2. J.P. Euzéby. "Natronomonas". List of Prokaryotic names with Standing in Nomenclature (LPSN). Retrieved 2023-10-10.
3. "The LTP". Retrieved 20 November 2023.
4. "LTP_all tree in newick format". Retrieved 20 November 2023.
5. "LTP_08_2023 Release Notes" (PDF). Retrieved 20 November 2023.
6. "GTDB release 08-RS214". Genome Taxonomy Database. Retrieved 10 May 2023.
7. "ar53_r214.sp_label". Genome Taxonomy Database. Retrieved 10 May 2023.
8. "Taxon History". Genome Taxonomy Database. Retrieved 10 May 2023.

Further reading

Scientific journals

• Oren A; Ventosa A (2000). "International Committee on Systematic Bacteriology Subcommittee on the taxonomy of Halobacteriaceae. Minutes of the meetings, 16 August 1999, Sydney, Australia". Int. J. Syst. Evol. Microbiol. 50 (3): 1405–1407. doi:10.1099/00207713-50-3-1405. PMID 10843089.
• Kamekura M; Dyall-Smith ML; Upasani V; Ventosa A; et al. (1997). "Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba, and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively". Int. J. Syst. Bacteriol. 47 (3): 853–857. doi:10.1099/00207713-47-3-853. PMID 9226918.
• Natarajan, Janani; Schultz, Anita; Kurz, Ursula; Schultz, Joachim E. (2014). "Biochemical characterization of the tandem HAMP domain from Natronomonas pharaonis as an intraprotein signal transducer". FEBS Journal. 281 (14): 3218–3227. doi:10.1111/febs.12855. PMID 24863503.

Scientific books

• Gibbons, NE (1974). "Family V. Halobacteriaceae fam. nov.". In RE Buchanan; NE Gibbons (eds.). Bergey's Manual of Determinative Bacteriology (8th ed.). Baltimore: The Williams & Wilkins Co. ISBN 978-0-683-01117-3.

External links

• Type strain of Natronomonas pharaonis at BacDive - the Bacterial Diversity Metadatabase

• Archaea genera
• Taxa described in 1997