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SMC3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2WD5
Identifiers
Aliases	SMC3, BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1, structural maintenance of chromosomes 3
External IDs	OMIM: 606062; MGI: 1339795; HomoloGene: 3974; GeneCards: SMC3; OMA:SMC3 - orthologs
Gene location (Human) Chr. Chromosome 10 (human) Band 10q25.2 Start 110,567,684 bp End 110,606,048 bp
Gene location (Mouse) Chr. Chromosome 19 (mouse) Band 19|19 D2 Start 53,588,829 bp End 53,634,264 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in tendon of biceps brachii ventricular zone oocyte testicle gonad ganglionic eminence internal globus pallidus Achilles tendon secondary oocyte superior surface of tongue Top expressed in genital tubercle tail of embryo fetal liver hematopoietic progenitor cell ventricular zone mandibular prominence maxillary prominence epiblast hand secondary oocyte somite More reference expression data BioGPS More reference expression data
Gene ontology Molecular function microtubule motor activity nucleotide binding mediator complex binding chromatin binding protein binding protein heterodimerization activity ATP binding dynein complex binding beta-tubulin binding protein-containing complex binding Cellular component cytosol meiotic cohesin complex nuclear matrix chromosome nucleoplasm lateral element chromatin chromosome, centromeric region nucleus basement membrane intracellular anatomical structure cohesin complex mitotic spindle pole Biological process regulation of DNA replication chromosome organization cellular response to DNA damage stimulus stem cell population maintenance cell division negative regulation of DNA endoreduplication cell cycle DNA repair sister chromatid cohesion mitotic cell cycle meiosis regulation of mitotic spindle assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9126 13006 Ensembl ENSG00000108055 ENSMUSG00000024974 UniProt Q9UQE7 Q9CW03 RefSeq (mRNA) NM_005445 NM_007790 RefSeq (protein) NP_005436 NP_031816 Location (UCSC) Chr 10: 110.57 – 110.61 Mb Chr 19: 53.59 – 53.63 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Structural maintenance of chromosomes protein 3 (SMC3) is a protein that in humans is encoded by the SMC3 gene. SMC3 is a subunit of the Cohesin complex which mediates sister chromatid cohesion, homologous recombination and DNA looping. Cohesin is formed of SMC3, SMC1, RAD21 and either SA1 or SA2. In humans, SMC3 is present in all cohesin complexes whereas there are multiple paralogs for the other subunits.

SMC3 is a member of the SMC protein family. Members of this family are key regulators of DNA repair, chromosome condensation and chromosome segregation.

Structure and interactions

The domain organisation of SMC proteins is evolutionarily conserved and is composed of an N-terminal Walker A motif, coiled-coil, "hinge", coiled-coil and a C-terminal Walker B motif. The protein folds back on itself to form a rod-shaped molecule with a heterodimerisation "hinge" domain at one end and an ABC-type ATPase "head" at the other. These globular domains are separated by a ~50 nm anti-parallel coiled-coil. SMC3 and SMC1 bind via their hinge domains creating V-shaped heterodimers. The N-terminal domain of RAD21 binds to the coiled coil of SMC3 just above the head domain while the C-terminal domain of RAD21 binds the head domain of SMC1. This end to end binding of the SMC3-SMC1-RAD21 trimer creates a closed ring within which DNA can be entrapped. SA1 or

When DNA is replicated and sister chromatid cohesion is established SMC3 is acetylated on a pair of highly conserved lysines by ESCO1 and ESCO2. In budding yeast this modification is sufficient to stabilise cohesin on the DNA until mitosis but in animals, binding of sororin is also required.

During meiosis, SMC3 forms cohesin complexes with SMC1ß, STAG3 and REC8 which generate cohesion between homologous chromosomes and sister chromatids.

Cornelia de Lange syndrome

Cornelia de Lange syndrome (CdLS) is a rare genetic disorder that presents with variable clinical abnormalities including dysmorphic features, severe growth retardation, global developmental delay, and intellectual disability. SMC3 is one of five genes that have been implicated in CdLS. In one case report, a novel SMC3 gene duplication was detected in a child with failure to thrive, hypotonia and facial dysmorphic features of CdLS. The same duplication was also observed in the mother, who had milder dysmorphic facies.

Role in Basement Membrane

SMC3 occurs in certain cell types as a secreted protein and post-translational addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.

References

1. ^ GRCh38: Ensembl release 89: ENSG00000108055 – Ensembl, May 2017
2. ^ GRCm38: Ensembl release 89: ENSMUSG00000024974 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. ^ "Entrez Gene: SMC3 structural maintenance of chromosomes 3".
6. Garcia-Cruz R, Brieño MA, Roig I, Grossmann M, Velilla E, Pujol A, Cabero L, Pessarrodona A, Barbero JL, Garcia Caldés M (2010). "Dynamics of cohesin proteins REC8, STAG3, SMC1 beta and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes". Hum. Reprod. 25 (9): 2316–27. doi:10.1093/humrep/deq180. PMID 20634189.
7. ^ Infante E, Alkorta-Aranburu G, El-Gharbawy A (2017). "Rare form of autosomal dominant familial Cornelia de Lange syndrome due to a novel duplication in SMC3". Clinical Case Reports. 5 (8): 1277–1283. doi:10.1002/ccr3.1010. PMC 5538066. PMID 28781842.

External links

• GeneReviews/NCBI/UW/NIH entry on Cornelia de Lange syndrome
• FactorBook SMC3

• Genes on human chromosome 10
• Human proteins
• Genes mutated in mice