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An antimicrobial is an agent that kills microorganisms (microbicide) or stops their growth (bacteriostatic agent). Antimicrobial medicines can be grouped according to the microorganisms they act primarily against. For example, antibiotics are used against bacteria, and antifungals are used against fungi. They can also be classified according to their function. Antimicrobial medicines to treat infection are known as ⠀⠀antimicrobial chemotherapy, while antimicrobial drugs are used to prevent infection, which known as antimicrobial prophylaxis.

The main classes of antimicrobial agents are disinfectants (non-selective agents, such as bleach), which kill a wide range of microbes on non-living surfaces to prevent the spread of illness, antiseptics (which are applied to living tissue and help reduce infection during surgery), and antibiotics (which destroy microorganisms within the body). The term antibiotic originally described only those formulations derived from living microorganisms but is now also applied to synthetic agents, such as sulfonamides or fluoroquinolones. Though the term used to be restricted to antibacterial (and is often used as a synonym for them by medical professionals and in medical literature), its context has broadened to include all antimicrobials. Antibacterial agents can be further subdivided into bactericidal agents, which kill bacteria, and bacteriostatic agents, which slow down or stall bacterial growth. In response, further advancements in antimicrobial technologies have resulted in solutions that can go beyond simply inhibiting microbial growth. Instead, certain types of porous media have been developed to kill microbes on contact. The misuse and overuse of antimicrobials in humans, animals and plants are the main drivers in the development of drug-resistant pathogens. It is estimated that bacterial antimicrobial resistance (AMR) was directly responsible for 1.27 million global deaths in 2019 and contributed to 4.95 million deaths.

History

Antimicrobial use has been common practice for at least 2000 years. Ancient Egyptians and ancient Greeks used specific molds and plant extracts to treat infection.

In the 19th century, microbiologists such as Louis Pasteur and Jules Francois Joubert observed antagonism between some bacteria and discussed the merits of controlling these interactions in medicine. Louis Pasteur's work in fermentation and spontaneous generation led to the distinction between anaerobic and aerobic bacteria. The information garnered by Pasteur led Joseph Lister to incorporate antiseptic methods, such as sterilizing surgical tools and debriding wounds into surgical procedures. The implementation of these antiseptic techniques drastically reduced the number of infections and subsequent deaths associated with surgical procedures. Louis Pasteur's work in microbiology also led to the development of many vaccines for life-threatening diseases such as anthrax and rabies. On September 3, 1928, Alexander Fleming returned from a vacation and discovered that a Petri dish filled with Staphylococcus was separated into colonies due to the antimicrobial fungus Penicillium rubens. Fleming and his associates struggled to isolate the antimicrobial but referenced its therapeutic potential in 1929 in the British Journal of Experimental Pathology. In 1942, Howard Florey, Ernst Chain, and Edward Abraham used Fleming's work to purify and extract penicillin for medicinal uses earning them the 1945 Nobel Prize in Medicine.

Chemical

Antibacterials

Antibacterials are used to treat bacterial infections. Antibiotics are classified generally as beta-lactams, macrolides, quinolones, tetracyclines or aminoglycosides. Their classification within these categories depends on their antimicrobial spectra, pharmacodynamics, and chemical composition. Prolonged use of certain antibacterials can decrease the number of enteric bacteria, which may have a negative impact on health. Consumption of probiotics and reasonable eating may help to replace destroyed gut flora. Stool transplants may be considered for patients who are having difficulty recovering from prolonged antibiotic treatment, as for recurrent Clostridioides difficile infections.

The discovery, development and use of antibacterials during the 20th century have reduced mortality from bacterial infections. The antibiotic era began with the therapeutic application of sulfonamide drugs in 1936, followed by a "golden" period of discovery from about 1945 to 1970, when a number of structurally diverse and highly effective agents were discovered and developed. Since 1980, the introduction of new antimicrobial agents for clinical use has declined, in part because of the enormous expense of developing and testing new drugs. In parallel, there has been an alarming increase in antimicrobial resistance of bacteria, fungi, parasites and some viruses to multiple existing agents.

Antibacterials are among the most commonly used drugs and among the drugs commonly misused by physicians, for example, in viral respiratory tract infections. As a consequence of widespread and injudicious use of antibacterials, there has been an accelerated emergence of antibiotic-resistant pathogens, resulting in a serious threat to global public health. The resistance problem demands that a renewed effort be made to seek antibacterial agents effective against pathogenic bacteria resistant to current antibacterials. Possible strategies towards this objective include increased sampling from diverse environments and application of metagenomics to identify bioactive compounds produced by currently unknown and uncultured microorganisms as well as the development of small-molecule libraries customized for bacterial targets.

Antifungals

Antifungals are used to kill or prevent further growth of fungi. In medicine, they are used as a treatment for infections such as athlete's foot, ringworm and thrush and work by exploiting differences between mammalian and fungal cells. Unlike bacteria, both fungi and humans are eukaryotes. Thus, fungal and human cells are similar at the molecular level, making it more difficult to find a target for an antifungal drug to attack that does not also exist in the host organism. Consequently, there are often side effects to some of these drugs. Some of these side effects can be life-threatening if the drug is not used properly.

As well as their use in medicine, antifungals are frequently sought after to control indoor mold in damp or wet home materials. Sodium bicarbonate (baking soda) blasted on to surfaces acts as an antifungal. Another antifungal solution applied after or without blasting by soda is a mix of hydrogen peroxide and a thin surface coating that neutralizes mold and encapsulates the surface to prevent spore release. Some paints are also manufactured with an added antifungal agent for use in high humidity areas such as bathrooms or kitchens. Other antifungal surface treatments typically contain variants of metals known to suppress mold growth e.g. pigments or solutions containing copper, silver or zinc. These solutions are not usually available to the general public because of their toxicity.

Antivirals

Antiviral drugs are a class of medication used specifically for treating viral infections. Like antibiotics, specific antivirals are used for specific viruses. They should be distinguished from viricides, which actively deactivate virus particles outside the body.

Many antiviral drugs are designed to treat infections by retroviruses, including HIV. Important antiretroviral drugs include the class of protease inhibitors. Herpes viruses, best known for causing cold sores and genital herpes, are usually treated with the nucleoside analogue acyclovir. Viral hepatitis is caused by five unrelated hepatotropic viruses (A-E) and may be treated with antiviral drugs depending on the type of infection. Some influenza A and B viruses have become resistant to neuraminidase inhibitors such as oseltamivir, and the search for new substances continues.

Antiparasitics

Antiparasitics are a class of medications indicated for the treatment of infectious diseases such as leishmaniasis, malaria and Chagas disease, which are caused by parasites such as nematodes, cestodes, trematodes and infectious protozoa. Antiparasitic medications include metronidazole, iodoquinol and albendazole. Like all therapeutic antimicrobials, they must kill the infecting organism without serious damage to the host.

Broad-spectrum therapeutics

Broad-spectrum therapeutics are active against multiple classes of pathogens. Such therapeutics have been suggested as potential emergency treatments for pandemics.

Non-pharmaceutical

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A wide range of chemical and natural compounds are used as antimicrobials. Organic acids and their salts are used widely in food products, e.g. lactic acid, citric acid, acetic acid, either as ingredients or as disinfectants. For example, beef carcasses often are sprayed with acids, and then rinsed or steamed, to reduce the prevalence of Escherichia coli.

Heavy metal cations such as Hg and Pb have antimicrobial activities, but can be toxic. In recent years, the antimicrobial activity of coordination compounds has been investigated.

Traditional herbalists used plants to treat infectious disease. Many of these plants have been investigated scientifically for antimicrobial activity, and some plant products have been shown to inhibit the growth of pathogenic microorganisms. A number of these agents appear to have structures and modes of action that are distinct from those of the antibiotics in current use, suggesting that cross-resistance with agents already in use may be minimal.

Copper

Copper-alloy surfaces have natural intrinsic antimicrobial properties and can kill microorganisms such as E. coli and Staphylococcus. The United States Environmental Protection Agency approved the registration of antimicrobial copper alloy surfaces for use in addition to regular cleaning and disinfection to control infections. Antimicrobial copper alloys are being installed in some healthcare facilities and subway transit systems as a public hygienic measure. Copper nanoparticles are attracting interest for the intrinsic antimicrobial behaviors.

Essential oils

Many essential oils included in herbal pharmacopoeias are claimed to possess antimicrobial activity, with the oils of bay, cinnamon, clove and thyme reported to be the most potent in studies with foodborne bacterial pathogens. Coconut oil is also known for its antimicrobial properties. Active constituents include terpenoids and secondary metabolites. Despite their prevalent use in alternative medicine, essential oils have seen limited use in mainstream medicine. While 25 to 50% of pharmaceutical compounds are plant-derived, none are used as antimicrobials, though there has been increased research in this direction. Barriers to increased usage in mainstream medicine include poor regulatory oversight and quality control, mislabeled or misidentified products, and limited modes of delivery.

Antimicrobial pesticides

According to the U.S. Environmental Protection Agency (EPA), and defined by the Federal Insecticide, Fungicide, and Rodenticide Act, antimicrobial pesticides are used to control growth of microbes through disinfection, sanitation, or reduction of development and to protect inanimate objects, industrial processes or systems, surfaces, water, or other chemical substances from contamination, fouling, or deterioration caused by bacteria, viruses, fungi, protozoa, algae, or slime. The EPA monitors products, such as disinfectants/sanitizers for use in hospitals or homes, to ascertain efficacy. Products that are meant for public health are therefore under this monitoring system, including products used for drinking water, swimming pools, food sanitation, and other environmental surfaces. These pesticide products are registered under the premise that, when used properly, they do not demonstrate unreasonable side effects to humans or the environment. Even once certain products are on the market, the EPA continues to monitor and evaluate them to make sure they maintain efficacy in protecting public health.

Public health products regulated by the EPA are divided into three categories:

• Disinfectants: Destroy or inactivate microorganisms (bacteria, fungi, viruses,) but may not act as sporicides (as those are the most difficult form to destroy). According to efficacy data, the EPA will classify a disinfectant as limited, general/broad spectrum, or as a hospital disinfectant.
• Sanitizers: Reduce the number of microorganisms, but may not kill or eliminate all of them.
• Sterilizers (Sporicides): Eliminate all bacteria, fungi, spores, and viruses.

Antimicrobial pesticide safety

Antimicrobial pesticides have the potential to be a major factor in drug resistance. Organizations such as the World Health Organization call for significant reduction in their use globally to combat this. According to a 2010 Centers for Disease Control and Prevention report, health-care workers can take steps to improve their safety measures against antimicrobial pesticide exposure. Workers are advised to minimize exposure to these agents by wearing personal protective equipment such as gloves and safety glasses. Additionally, it is important to follow the handling instructions properly, as that is how the EPA has deemed them as safe to use. Employees should be educated about the health hazards and encouraged to seek medical care if exposure occurs.

Ozone

Ozone can kill microorganisms in air, water and process equipment and has been used in settings such as kitchen exhaust ventilation, garbage rooms, grease traps, biogas plants, wastewater treatment plants, textile production, breweries, dairies, food and hygiene production, pharmaceutical industries, bottling plants, zoos, municipal drinking-water systems, swimming pools and spas, and in the laundering of clothes and treatment of in–house mold and odors.

Antimicrobial scrubs

Antimicrobial scrubs can reduce the accumulation of odors and stains on scrubs, which in turn improves their longevity. These scrubs also come in a variety of colors and styles. As antimicrobial technology develops at a rapid pace, these scrubs are readily available, with more advanced versions hitting the market every year. These bacteria could then be spread to office desks, break rooms, computers, and other shared technology. This can lead to outbreaks and infections like MRSA, treatments for which cost the healthcare industry $20 billion a year.

Halogens

Elements such as chlorine, iodine, fluorine, and bromine are nonmetallic in nature and constitute the halogen family. Each of these halogens have a different antimicrobial effect that is influenced by various factors such as pH, temperature, contact time, and type of microorganism. Chlorine and iodine are the two most commonly used antimicrobials. Chlorine is extensively used as a disinfectant in the water treatment plants, drug, and food industries. In wastewater treatment plants, chlorine is widely used as a disinfectant. It oxidizes soluble contaminants and kills bacteria and viruses. It is also highly effective against bacterial spores. The mode of action is by breaking the bonds present in these microorganisms. When a bacterial enzyme comes in contact with a compound containing chlorine, the hydrogen atom in that molecule gets displaced and is replaced with chlorine. This thus changes the enzyme function which in turn leads to the death of the bacterium. Iodine is most commonly used for sterilization and wound cleaning. The three major antimicrobial compounds containing iodine are alcohol-iodine solution, an aqueous solution of iodine, and iodophors. Iodophors are more bactericidal and are used as antiseptics as they are less irritating when applied to the skin. Bacterial spores on the other hand cannot be killed by iodine, but they can be inhibited by iodophors. The growth of microorganisms is inhibited when iodine penetrates into the cells and oxidizes proteins, genetic material, and fatty acids. Bromine is also an effective antimicrobial that is used in water treatment plants. When mixed with chlorine it is highly effective against bacterial spores such as S. faecalis.

Alcohols

Alcohols are commonly used as disinfectants and antiseptics. Alcohols kill vegetative bacteria, most viruses and fungi. Ethyl alcohol, n-propanol and isopropyl alcohol are the most commonly used antimicrobial agents. Methanol is also a disinfecting agent but is not generally used as it is highly poisonous. Escherichia coli, Salmonella, and Staphylococcus aureus are a few bacteria whose growth can be inhibited by alcohols. Alcohols have a high efficiency against enveloped viruses (60–70% ethyl alcohol) 70% isopropyl alcohol or ethanol are highly effective as an antimicrobial agent. In the presence of water, 70% alcohol causes coagulation of the proteins thus inhibiting microbial growth. Alcohols are not quite efficient when it comes to spores. The mode of action is by denaturing the proteins. Alcohols interfere with the hydrogen bonds present in the protein structure. Alcohols also dissolve the lipid membranes that are present in microorganisms. Disruption of the cell membrane is another property of alcohols that aid in cell death. Alcohols are cheap and effective antimicrobials. They are widely used in the pharmaceutical industry.  Alcohols are commonly used in hand sanitizers, antiseptics, and disinfectants.

Phenol and Phenolic compounds

Phenol also known as carbolic acid was one of the first chemicals which was used as an antimicrobial agent. It has high antiseptic properties. It is bacteriostatic at concentrations of 0.1%–1% and is bactericidal/fungicidal at 1%–2%. A 5% solution kills anthrax spores in 48 hr. Phenols are most commonly used in oral mouth washes and household cleaning agents. They are active against a wide range of bacteria, fungi and viruses.  Today phenol derivatives such as thymol and cresol are used because they are less toxic compared to phenol. These phenolic compounds have a benzene ring along with the –OH group incorporated into their structures. They have a higher antimicrobial activity. These compounds inhibit microbial growth by precipitating proteins which lead to their denaturation and by penetrating into the cell membrane of microorganisms and disrupting it. Phenolic compounds can also deactivate enzymes and damage the amino acids in microbial cells. Phenolics such as fentichlore, an antibacterial and antifungal agent are used as an oral treatment for fungal infections. Trischlosan is highly effective against both gram-positive and gram-negative bacteria. Hexachlorophene (Bisphenol) is used as a surfactant. It is widely used in soaps, handwashes, and skin products because of its antiseptic properties. It is also used as a sterilizing agent. Cresol is an effective antimicrobial and is widely used in mouthwashes and cough drops. Phenolics have high antimicrobial activity against bacteria such as Staphylococcus epidermidis and Pseudomonas aeruginosa. 2-Phenylphenol-water solutions are used in immersion treatments of fruit for packing. (It is not used on the packing materials however.) Ihloff and Kalitzki 1961 find a small but measurable amount remains in the skin of fruits processed in this manner.

Aldehydes

They are highly effective against bacteria, fungi, and viruses.  Aldehydes inhibit bacterial growth by disrupting the outer membrane. They are used in the disinfection and sterilization of surgical instruments. Being highly toxic they are not used in antiseptics. Currently, only three aldehyde compounds are of widespread practical use as disinfectant biocides, namely glutaraldehyde, formaldehyde, and ortho-phthalaldehyde (OPA) despite the demonstration that many other aldehydes possess good antimicrobial activity. However, due to its long contact time other disinfectants are commonly preferred.

Physical

Heat

Microorganisms have a minimum temperature, an optimum, and a maximum temperature for growth. High temperature as well as low temperatures are used as physical agents of control. Different organisms show different degrees of resistance or susceptibility to heat or temperature, some organisms such as bacterial endospore are more resistant while vegetative cells are less resistant and are easily killed at lower temperatures. Another method that involves the use of heat to kill microorganisms is fractional sterilization. This process involves the exposure to a temperature of 100 degrees Celsius for an hour, on each for several days. Fractional sterilization is also called tyndallization. Bacterial endospores can be killed using this method. Both dry and moist heat are effective in eliminating microbial life. For example, jars used to store preserves such as jam can be sterilized by heating them in a conventional oven. Heat is also used in pasteurization, a method for slowing the spoilage of foods such as milk, cheese, juices, wines and vinegar. Such products are heated to a certain temperature for a set period of time, which greatly reduces the number of harmful microorganisms. Low temperature is also used to inhibit microbial activity by slowing down microbial metabolism.

Radiation

Foods are often irradiated to kill harmful pathogens. There are two types of radiations that are used to inhibit the growth of microorganisms – ionizing and non-ionizing radiations. Common sources of radiation used in food sterilization include cobalt-60 (a gamma emitter), electron beams and X-rays. Ultraviolet light is also used to disinfect drinking water, both in small-scale personal-use systems and larger-scale community water purification systems.

Desiccation

Desiccation is also known as dehydration.  It is the state of extreme dryness or the process of extreme drying. Some microorganisms like bacteria, yeasts and molds require water for their growth. Desiccation dries up the water content thus inhibiting microbial growth. On the availability of water, the bacteria resume their growth, thus desiccation does not completely inhibit bacterial growth. The instrument used to carry out this process is called a desiccator. This process is widely used in the food industry and is an efficient method for food preservation. Desiccation is also largely used in the pharmaceutical industry to store vaccines and other products.

Antimicrobial surfaces

Antimicrobial surfaces are designed to either inhibit the ability of microorganisms to grow or damaging them by chemical (copper toxicity) or physical processes (micro/nano-pillars to rupture cell walls). These surfaces are especially important for the healthcare industry. Designing effective antimicrobial surfaces demands an in-depth understanding of the initial microbe-surface adhesion mechanisms. Molecular dynamics simulation and time-lapse imaging are typically used to investigate these mechanisms.

Osmotic pressure

Osmotic pressure is the pressure required to prevent a solvent from passing from a region of high concentration to a region of low concentration through a semipermeable membrane.  When the concentration of dissolved materials or solute is higher inside the cell than it is outside, the cell is said to be in a hypotonic environment and water will flow into the cell.When the bacteria is placed in hypertonic solution, it causes plasmolysis or cell shrinking, similarly in hypotonic solution, bacteria undergoes plasmotysis or turgid state. This plasmolysis and plasmotysis kills bacteria because it causes change in osmotic pressure.

Antimicrobial resistance

Antimicrobial resistance The misuse and overuse of antimicrobials in humans, animals and plants are the main drivers in the development of drug-resistant pathogens. It is estimated that bacterial antimicrobial resistance (AMR) was directly responsible for 1.27 million global deaths in 2019 and contributed to 4.95 million deaths.

See also

• Biocide
• Antiviral drug

References

1. "Antimicrobial". Merriam-Webster Online Dictionary. Archived from the original on 24 April 2009. Retrieved 2009-05-02.
2. Leekha, Surbhi; Terrell, Christine L.; Edson, Randall S. (February 2011). "General Principles of Antimicrobial Therapy". Mayo Clinic Proceedings. 86 (2): 156–167. doi:10.4065/mcp.2010.0639. PMC 3031442. PMID 21282489.
3. "Antimicrobial Porous Media | Microbicidal Technology | Porex Barrier Technology". www.porex.com. Archived from the original on 2017-03-03. Retrieved 2017-02-16.
4. ^ "Antimicrobial resistance". who.int. World Health Organization. 21 November 2023. Retrieved 11 October 2024.
5. Wainwright M (1989). "Moulds in ancient and more recent medicine". Mycologist. 3 (1): 21–23. doi:10.1016/S0269-915X(89)80010-2.
6. Kingston W (June 2008). "Irish contributions to the origins of antibiotics". Irish Journal of Medical Science. 177 (2): 87–92. doi:10.1007/s11845-008-0139-x. PMID 18347757. S2CID 32847260.
7. Ullmann A (23 Dec 2019). "Louis Pasteur | Biography, Inventions, Achievements, & Facts". Encyclopedia Britannica. Encyclopedia Britannica, inc. Retrieved 24 February 2020.
8. Fleming A (1929). "On the Antibacterial Action of Cultures of a Penicillium, with Special Reference to their use in the Isolation of B. influenzae". The British Journal of Experimental Pathology. 10 (3): 226–236.
9. "The Nobel Prize in Physiology or Medicine 1945". The Nobel Prize Organization.
10. ^ Gilbert DN, Saag MS (2018). Sanford Guide to Antimicrobial Therapy (48th ed.). Antimicrobial Therapy Incorporated. ISBN 978-1944272067.
11. Brandt LJ (February 2013). "American Journal of Gastroenterology Lecture: Intestinal microbiota and the role of fecal microbiota transplant (FMT) in treatment of C. difficile infection". The American Journal of Gastroenterology. 108 (2): 177–85. doi:10.1038/ajg.2012.450. PMID 23318479. S2CID 5843938.
12. Kellermayer R (November 2013). "Prospects and challenges for intestinal microbiome therapy in pediatric gastrointestinal disorders". World Journal of Gastrointestinal Pathophysiology. 4 (4): 91–3. doi:10.4291/wjgp.v4.i4.91. PMC 3829459. PMID 24244876.
13. Ventola CL (April 2015). "The antibiotic resistance crisis: part 1: causes and threats". P & T. 40 (4): 277–83. PMC 4378521. PMID 25859123.
14. Tanwar J, Das S, Fatima Z, Hameed S (Jul 16, 2014). "Multidrug resistance: an emerging crisis". Interdisciplinary Perspectives on Infectious Diseases. 2014: 541340. doi:10.1155/2014/541340. PMC 4124702. PMID 25140175.
15. Committee on New Directions in the Study of Antimicrobial Therapeutics (2006). "Challenges for the Development of New Antimicrobials— Rethinking the Approaches". Challenges for the Development of New Antibiotics — Rethinking the Approaches. National Academies Press. NBK19843.
16. Houšť, Jiří; Spížek, Jaroslav; Havlíček, Vladimír (2020-03-12). "Antifungal Drugs". Metabolites. 10 (3): 106. doi:10.3390/metabo10030106. ISSN 2218-1989. PMC 7143493. PMID 32178468.
17. Malandrakis, Anastasios A.; Kavroulakis, Nektarios; Chrysikopoulos, Constantinos V. (June 2019). "Use of copper, silver and zinc nanoparticles against foliar and soil-borne plant pathogens". Science of the Total Environment. 670: 292–299. Bibcode:2019ScTEn.670..292M. doi:10.1016/j.scitotenv.2019.03.210. PMID 30903901. S2CID 85460203.
18. Arakawa, Tsutomu; Yamasaki, Hisashi; Ikeda, Keiko; Ejima, Daisuke; Naito, Takeshi; Koyama, A. Hajime (2009). "Antiviral and Virucidal Activities of Natural Products". Current Medicinal Chemistry. 16 (20): 2485–2497. doi:10.2174/092986709788682065. PMID 19601794.
19. Lampejo, Temi (July 2020). "Influenza and antiviral resistance: an overview". European Journal of Clinical Microbiology & Infectious Diseases. 39 (7): 1201–1208. doi:10.1007/s10096-020-03840-9. ISSN 0934-9723. PMC 7223162. PMID 32056049.
20. Pink, Richard; Hudson, Alan; Mouriès, Marie-Annick; Bendig, Mary (September 2005). "Opportunities and Challenges in Antiparasitic Drug Discovery". Nature Reviews Drug Discovery. 4 (9): 727–740. doi:10.1038/nrd1824. ISSN 1474-1784. PMID 16138106. S2CID 19379800.
21. Firth, Anton; Prathapan, Praveen (2021-01-01). "Broad-spectrum therapeutics: A new antimicrobial class". Current Research in Pharmacology and Drug Discovery. 2: 100011. doi:10.1016/j.crphar.2020.100011. ISSN 2590-2571. PMC 8035643. PMID 34870144.
22. Castillo, A.; Lucia, L.M.; Roberson, D.B.; Stevenson, T.H.; Mercado, I.; Acuff, G.R. (January 2001). "Lactic Acid Sprays Reduce Bacterial Pathogens on Cold Beef Carcass Surfaces and in Subsequently Produced Ground Beef". Journal of Food Protection. 64 (1): 58–62. doi:10.4315/0362-028X-64.1.58. PMID 11198442.
23. Ratia, Carlos; Soengas, Raquel G.; Soto, Sara M. (2022). "Gold-Derived Molecules as New Antimicrobial Agents". Frontiers in Microbiology. 13: 846959. doi:10.3389/fmicb.2022.846959. ISSN 1664-302X. PMC 8984462. PMID 35401486.
24. Pintus, Anna; Aragoni, M. Carla; Cinellu, Maria A.; Maiore, Laura; Isaia, Francesco; Lippolis, Vito; Orrù, Germano; Tuveri, Enrica; Zucca, Antonio; Arca, Massimiliano (May 2017). "[Au(pyb-H)(mnt)]: A novel gold(III) 1,2-dithiolene cyclometalated complex with antimicrobial activity (pyb-H=C-deprotonated 2-benzylpyridine; mnt=1,2-dicyanoethene-1,2-dithiolate)". Journal of Inorganic Biochemistry. 170: 188–194. doi:10.1016/j.jinorgbio.2017.02.015. ISSN 1873-3344. PMID 28260677.
25. Marques, Fernanda; Sousa, Sílvia A.; Leitão, Jorge H.; Morais, Tânia S.; Le Gal, Yann; Lorcy, Dominique (2021-04-01). "Gold(III) bisdithiolate complexes: molecular conductors that also exhibit anticancer and antimicrobial activities". Annals of Medicine. 53 (sup1): S29–S30. doi:10.1080/07853890.2021.1896913. ISSN 0785-3890. PMC 8480714.
26. Podda, Enrico; Arca, Massimiliano; Atzeni, Giulia; Coles, Simon J.; Ibba, Antonella; Isaia, Francesco; Lippolis, Vito; Orrù, Germano; Orton, James B.; Pintus, Anna; Tuveri, Enrica (2020-04-28). "Antibacterial Activity of Amidodithiophosphonato Nickel(II) Complexes: An Experimental and Theoretical Approach". Molecules. 25 (9): 2052. doi:10.3390/molecules25092052. ISSN 1420-3049. PMC 7248947. PMID 32354035.
27. ^ Berida T, Adekunle Y, Dada-Adegbola H, Kdimy A, Roy S, Sarker S (May 2024). "Plant antibacterials: The challenges and opportunities". Heliyon. 10 (10): e31145. Bibcode:2024Heliy..1031145B. doi:10.1016/j.heliyon.2024.e31145. PMC 11128932. PMID 38803958.
28. Mollazadeh Moghaddam K, Arfan M, Rafique J, Rezaee S, Jafari Fesharaki P, Gohari AR, Shahverdi AR (September 2010). "The antifungal activity of Sarcococca saligna ethanol extract and its combination effect with fluconazole against different resistant Aspergillus species". Applied Biochemistry and Biotechnology. 162 (1): 127–33. doi:10.1007/s12010-009-8737-2. PMID 19685213. S2CID 8211327.
29. "Copper Touch Surfaces". Archived from the original on 2012-07-23. Retrieved 2011-09-27.
30. ^ Morrison, Jim (14 April 2020). "Copper's Virus-Killing Powers Were Known Even to the Ancients". Smithsonian Magazine. Archived from the original on 20 March 2023. Retrieved 2 April 2023.
31. "EPA Registers Copper Surfaces for Residual Use Against Coronavirus" (Press release). Washington, DC: EPA Press Office. 10 February 2021. Archived from the original on 22 March 2023. Retrieved 2 April 2023.
32. Ermini, Maria Laura; Voliani, Valerio (2021-04-01). "Antimicrobial Nano-Agents: The Copper Age". ACS Nano. 15 (4): 6008–6029. doi:10.1021/acsnano.0c10756. ISSN 1936-0851. PMC 8155324. PMID 33792292.
33. Smith-Palmer A, Stewart J, Fyfe L (February 1998). "Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens". Letters in Applied Microbiology. 26 (2): 118–22. doi:10.1046/j.1472-765x.1998.00303.x. PMID 9569693. S2CID 39803630.
34. Kalemba D, Kunicka A (May 2003). "Antibacterial and antifungal properties of essential oils". Current Medicinal Chemistry. 10 (10): 813–29. doi:10.2174/0929867033457719. PMID 12678685.
35. Blimie Wassertheil (Feb 5, 2018). "Beyond the Bowl of Chicken Soup". Binah. pp. 46–50.
36. Schnitzler, Paul (2019). "Essential Oils for the Treatment of Herpes Simplex Virus Infections". Chemotherapy. 64 (1): 1–7. doi:10.1159/000501062. ISSN 0009-3157. PMID 31234166. S2CID 195356798.
37. Astani, Akram; Reichling, Jürgen; Schnitzler, Paul (May 2010). "Comparative study on the antiviral activity of selected monoterpenes derived from essential oils: Antiviral Activity of Monoterpenes Derived FromEssential Oils". Phytotherapy Research. 24 (5): 673–679. doi:10.1002/ptr.2955. PMC 7167768. PMID 19653195.
38. Cowan MM (October 1999). "Plant products as antimicrobial agents". Clinical Microbiology Reviews. 12 (4): 564–82. doi:10.1128/CMR.12.4.564. PMC 88925. PMID 10515903.
39. Chouhan, Sonam; Sharma, Kanika; Guleria, Sanjay (2017-08-08). "Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives". Medicines. 4 (3): 58. doi:10.3390/medicines4030058. ISSN 2305-6320. PMC 5622393. PMID 28930272.
40. ^ "What Are Antimicrobial Pesticides?". U.S. Environmental Protection Agency. 2000. Archived from the original on 2013-05-20. Retrieved 2013-05-05.
41. Sanders FT (2003). "The Role of the EPA in the Regulation of Antimicrobial Pesticides in the United States". Pesticide Outlook. 14 (2): 251–255. doi:10.1039/b314854h.
42. Sanders, Frank T. (2003-02-05). "The role of the EPA in the regulation of antimicrobial pesticides in the United States". Pesticide Outlook. 14 (6): 251–255. doi:10.1039/B314854H. ISSN 1465-8933.
43. Taneja, Neelam; Sharma, Megha (2019). "Antimicrobial resistance in the environment: The Indian scenario". Indian Journal of Medical Research. 149 (2): 119–120. doi:10.4103/ijmr.IJMR_331_18. PMC 6563737. PMID 31219076.
44. "World leaders and experts call for significant reduction in the use of antimicrobial drugs in global food systems". WHO Departmental News. Geneva, Nairobi, Paris, Rome. 2021-08-24. Retrieved 2022-04-18.
45. Centers for Disease Control Prevention (CDC) (May 2010). "Acute antimicrobial pesticide-related illnesses among workers in health-care facilities – California, Louisiana, Michigan, and Texas, 2002–2007". MMWR. Morbidity and Mortality Weekly Report. 59 (18): 551–6. PMID 20467413.
46. Habibi Najafi, Mohammad B.; Haddad Khodaparast, M. H. (2009-01-01). "Efficacy of ozone to reduce microbial populations in date fruits". Food Control. 20 (1): 27–30. doi:10.1016/j.foodcont.2008.01.010. ISSN 0956-7135.
47. Li, Chih-Shan; Wang, Yu-Chun (July 2003). "Surface Germicidal Effects of Ozone for Microorganisms". AIHA Journal. 64 (4): 533–537. doi:10.1080/15428110308984851. ISSN 1542-8117. PMID 12908871.
48. Prestinaci F, Pezzotti P, Pantosti A (October 2015). "Antimicrobial resistance: a global multifaceted phenomenon". Pathogens and Global Health. 109 (7): 309–18. doi:10.1179/2047773215Y.0000000030. PMC 4768623. PMID 26343252.
49. Odlaug, Theron E. (August 1981). "Antimicrobial Activity of Halogens". Journal of Food Protection. 44 (8): 608–613. doi:10.4315/0362-028X-44.8.608. PMID 30836538.
50. McDonnell, G.; Russell, A. D. (1999). "Antiseptics and Disinfectants: Activity, Action, and Resistance". Clinical Microbiology Reviews. 12 (1): 147–179. doi:10.1128/CMR.12.1.147. PMC 88911. PMID 9880479.
51. Saritha, Kongari; Rajesh, Angireddy; Manjulatha, Khanapur; Setty, Oruganti H.; Yenugu, Suresh (2015-06-09). "Mechanism of antibacterial action of the alcoholic extracts of Hemidesmus indicus (L.) R. Br. ex Schult, Leucas aspera (Wild.), Plumbago zeylanica L., and Tridax procumbens (L.) R. Br. ex Schult". Frontiers in Microbiology. 6: 577. doi:10.3389/fmicb.2015.00577. ISSN 1664-302X. PMC 4460426. PMID 26106379.
52. Ingólfsson, Helgi; Andersen, Olaf (August 2011). "Alcohol's Effects on Lipid Bilayer Properties". Biophysical Journal. 101 (4): 847–855. Bibcode:2011BpJ...101..847I. doi:10.1016/j.bpj.2011.07.013. PMC 3175087. PMID 21843475.
53. "Phenols and Related Compounds – Pharmacology".
54. Walsh, Danica J.; Livinghouse, Tom; Goeres, Darla M.; Mettler, Madelyn; Stewart, Philip S. (2019-10-01). "Antimicrobial Activity of Naturally Occurring Phenols and Derivatives Against Biofilm and Planktonic Bacteria". Frontiers in Chemistry. 7: 653. Bibcode:2019FrCh....7..653W. doi:10.3389/fchem.2019.00653. ISSN 2296-2646. PMC 6779693. PMID 31632948.
55. Lück, Erich (1997). Antimicrobial Food Additives : Characteristics · Uses · Effects. Berlin, Heidelberg: Springer Berlin Heidelberg. pp. XXIIX+260. ISBN 978-3-642-59202-7. OCLC 851702956.
56. "Aldehydes". Basicmedical Key. 9 May 2021.
57. ^ Pawel (21 November 2011). "Physical Agents to Control Microorganisms" (PDF). Uniwersytet Medyczny we Wrocławiu. Retrieved 13 November 2022.
58. "Physical agents to control microorganisms". 4 August 2017.
59. "Fractional sterilization".
60. "How Cold Does the Temperature Need to be to Kill Germs?".
61. "20467413". US EPA. Retrieved 28 October 2014.
62. "Physical agents to control microorganisms". 4 August 2017. Archived from the original on 2017-08-19.
63. "Irradiation of Food FAQ: What is the actual process of irradiation?". U.S. Centers for Disease Control and Prevention. Archived from the original on 20 April 2016. Retrieved 17 April 2016.
64. "UV Disinfection Drinking Water". Water Research Center. Retrieved 18 April 2016.
65. Ghaemmaghamian, Zahra; Zarghami, Reza; Walker, Gavin; O’Reilly, Emmet; Ziaee, Ahmad (2022-08-01). "Stabilizing vaccines via drying: Quality by design considerations". Advanced Drug Delivery Reviews. 187: 114313. doi:10.1016/j.addr.2022.114313. ISSN 0169-409X. PMID 35597307. S2CID 248934971.
66. Muller, M.P.; MacDougall, C.; Lim, M.; Armstrong, I.; Bialachowski, A.; Callery, S.; Ciccotelli, W.; Cividino, M.; Dennis, J.; Hota, S.; Garber, G.; Johnstone, J.; Katz, K.; McGeer, A.; Nankoosingh, V.; Richard, C.; Vearncombe, M. (2016-01-01). "Antimicrobial surfaces to prevent healthcare-associated infections: a systematic review". Journal of Hospital Infection. 92 (1): 7–13. doi:10.1016/j.jhin.2015.09.008. ISSN 0195-6701. PMID 26601608.
67. Sibilo, Rafaël; Mannelli, Ilaria; Reigada, Ramon; Manzo, Carlo; Noyan, Mehmet A.; Mazumder, Prantik; Pruneri, Valerio (2020-05-19). "Direct and Fast Assessment of Antimicrobial Surface Activity Using Molecular Dynamics Simulation and Time-Lapse Imaging". Analytical Chemistry. 92 (10): 6795–6800. doi:10.1021/acs.analchem.0c00367. ISSN 0003-2700. PMID 32295344.
68. "Physical agents to control microorganisms". 4 August 2017.

External links

• BURDEN of Resistance and Disease in European Nations – An EU-Project to estimate the financial burden of antibiotic resistance in European Hospitals
• Cochrane Wounds list of antimicrobials (PDF)
• https://courses.lumenlearning.com/microbiology/chapter/using-physical-methods-to-control-microorganis
• National Pesticide Information Center
• Overview of the use of Antimicrobials in plastic applications
• The Antimicrobial Index – A continuously updated list of antimicrobial agents found in scientific literature (includes plant extracts and peptides)

• Antimicrobials
• Biocides